Variations in national surveillance reporting for Mpox virus: A comparative analysis in 32 countries.

Objectives: Case Reporting and Surveillance (CRS) are crucial to combat the global spread of the Monkeypox virus (Mpox). To support CRS, the World Health Organization (WHO) has released standardized case definitions for suspected, probable, confirmed, and discarded cases. However, these definitions are often subject to localized adaptations by countries leading to heterogeneity in the collected data. Herein, we compared the differences in Mpox case definitions in 32 countries that collectively reported 96% of the global Mpox caseload. Methods: We extracted information regarding Mpox case definitions issued by the competent authorities in 32 included countries for suspected, probable, confirmed, and discarded cases. All data were gathered from online public sources. Results: For confirmed cases, 18 countries (56%) followed WHO guidelines and tested for Mpox using species specific PCR and/or sequencing. For probable and suspected cases, seven and eight countries, respectively were found to have not released definitions in their national documentations. Furthermore, none of the countries completely matched WHO's criteria for probable and suspected cases. Overlapping amalgamations of the criteria were frequently noticed. Regarding discarded cases, only 13 countries (41%) reported definitions, with only two countries (6%) having definition consistent with WHO guidelines. For case reporting, 12 countries (38%) were found to report both probable and confirmed cases, in line with WHO requirements. Conclusion: The heterogeneity in case definitions and reporting highlights the pressing need for homogenization in implementation of these guidelines. Homogenization would drastically improve data quality and aid data-scientists, epidemiologists, and clinicians to better understand and model the true disease burden in the society, followed by formulation and implementation of targeted interventions to curb the virus spread.

Large hospital-wide outbreak of Paenibacillus spp pseudobacteremia associated with contaminated nonsterile gloves.

We report a large, hospital-wide outbreak of pseudobacteremia by Paenibacillus spp. In total, 139 patients presented at least 1 positive blood culture during a 13-month period. Microbiological experiments indicated that contaminated nonsterile gloves were associated with the pseudobacteremia episodes. The outbreak was resolved by discontinuing the use of the involved brand.

Can domestic dogs be considered a good reservoir of Leishmania (L.) infantum chagasi in an endemic area of nonulcerated cutaneous leishmaniasis in Southern Honduras?

Dogs are considered to be the main domestic reservoir associated with the transmission of Leishmania (L.) infantum chagasi to humans in endemic areas of visceral leishmaniasis in America. However, little is known about the role of canines as a source of infection in endemic areas of nonulcerated cutaneous leishmaniasis (NUCL). Therefore, the objective of the present study was to investigate the role of dogs as a possible reservoir of the parasite in Southern Honduras. Dogs (n = 107) living with individuals affected by NUCL were clinically examined and biological material was collected for parasitological and immunological diagnosis. Most animals showed a healthy appearance and a few presented slight weight loss (64%), alopecia (7%), onychogryphosis (5%) and skin lesions (1%). The overall seroprevalence of Leishmania infection based on the DDP ® quick test and/or in-house ELISA serological test was 41%. The presence of the parasite's DNA was confirmed in 94% of the dogs; however, the average parasite load in the buffy coat was low at 6.09 parasites/µL, ranging between 0.221 and 50.2. The skin of seropositive dogs examined by histopathology using paraffin sections stained by hematoxylin and immunohistochemistry did not show cutaneous lesions or parasite amastigotes. Based on the absence of parasites in the skin and the low parasite load detected in the buffy coat, it seems that the dog does not represent a good source of infection for the vector in the endemic area of NUCL transmission in Southern Honduras. Other domestic and/or wild animals should be investigated.

Thymic alterations resulting from experimental visceral leishmaniasis in a Syrian hamster (Mesocricetus auratus).

BACKGROUND: The thymus is a lymphoid organ responsible for the development and maturation of T cells, which are part of the Th1, Th2, Th17, and Treg immune responses triggered by visceral leishmaniasis. The maturation and immunological development of T lymphocytes require a bidirectional interaction between the thymic microenvironment of epithelial cells, dendritic cells, and macrophages and the extracellular matrix with differentiating lymphocytes. OBJECTIVES: We evaluated the morphological characteristics and tissue distribution of hematopoietic and stromal cells in the thymuses of hamsters experimentally infected with Leishmania infantum, aiming to gain an insight into the pathophysiology of the disease. METHODS: Fifteen hamsters were subjected to intraperitoneal experimental infection with 107L. infantum promastigotes (MHOM/BR/1972/BH46). The animals were divided into three groups, each comprising five infected hamsters, and were then euthanized 15, 60, and 120 days postinfection. The control groups consisted of three groups of five healthy hamsters euthanized simultaneously with the infected ones. Thymic morphology was evaluated through histopathology and the cell composition through immunohistochemistry. We used antibodies to mark mesenchymal cells (anti-vimentin), epithelial cells (anti-cytokeratin), macrophages (anti-MAC387), B lymphocytes (anti-CD79a), and T lymphocytes (anti-CD3). Immunohistochemistry was also used to mark the parasite in the thymus. RESULTS: Infected and control hamsters showed no difference in thymic morphology and degree of atrophy. After 15 days of infection, CD3 + T lymphocytes in the thymus showed an increase that stabilized over time. At 120 days of infection, we detected a significant decrease in CD79a+ B lymphocytes. The parasite was present in the medullary and corticomedullary regions of 9 out of 15 hamsters. These findings confirm that the presence of a parasite can cause changes in a thymus cell population. However, further studies are needed to evaluate these changes' effects on the immune response of infected animals.

O-Linked Glycans of Candida albicans Interact with Specific GPCRs in the Coronary Endothelium and Inhibit the Cardiac Response to Agonists.

Candida albicans is an opportunistic fungal pathogen that may cause invasive infections in immunocompromised patients, disseminating through the bloodstream to other organs. In the heart, the initial step prior to invasion is the adhesion of the fungus to endothelial cells. Being the fungal cell wall's outermost structure and the first to come in contact with host cells, it greatly modulates the interplay that later will derive in the colonization of the host tissue. In this work, we studied the functional contribution of N-linked and O-linked mannans of the cell wall of C. albicans to the interaction with the coronary endothelium. An isolated rat heart model was used to assess cardiac parameters related to vascular and inotropic effects in response to phenylephrine (Phe), acetylcholine (aCh) and angiotensin II (Ang II) when treatments consisting of: (1) live and heat-killed (HK) C. albicans wild-type yeasts; (2) live C. albicans pmr1Δ yeasts (displaying shorter N-linked and O-linked mannans); (3) live C. albicans without N-linked and O-linked mannans; and (4) isolated N-linked and O-linked mannans were administered to the heart. Our results showed that C. albicans WT alters heart coronary perfusion pressure (vascular effect) and left ventricular pressure (inotropic effect) parameters in response to Phe and Ang II but not aCh, and these effects can be reversed by mannose. Similar results were observed when isolated cell walls, live C. albicans without N-linked mannans or isolated O-linked mannans were perfused into the heart. In contrast, C. albicans HK, C. albicans pmr1Δ, C. albicans without O-linked mannans or isolated N-linked mannans were not able to alter the CPP and LVP in response to the same agonists. Taken together, our data suggest that C. albicans interaction occurs with specific receptors on coronary endothelium and that O-linked mannan contributes to a greater extent to this interaction. Further studies are necessary to elucidate why specific receptors preferentially interact with this fungal cell wall structure.

Role of antigen-presenting cells in non-ulcerated skin lesions caused by Leishmania (Leishmania) infantum chagasi.

In Central America, infection by Leishmania (Leishmania) infantum chagasi causes visceral leishmaniasis and non-ulcerated cutaneous leishmaniasis (NUCL). This work aimed to evaluate the participation of subpopulations of antigen-presenting cells in skin lesions of patients affected by NUCL through double-staining immunohistochemistry using cellular and intracellular markers. Twenty-three skin biopsies from patients affected by NUCL were used. Histological sections stained by HE were used for histopathological study. Immunohistochemical studies were performed using primary antibodies against Langerhans cells, dermal dendritic cells, T lymphocytes, and the cytokines IL-12, IFN-γ, TNF-α, iNOS, and IL-10. The histopathological lesions were characterized by an inflammatory infiltrate, predominantly lymphohistiocytic, of variable intensity, with a diffuse arrangement associated with epithelioid granulomas and discreet parasitism. Double-staining immunohistochemistry showed higher participation of dendritic cells producing the proinflammatory cytokine IL-12 in relation to the other evaluated cytokines. Activation of the cellular immune response was marked by a higher density of CD8 Tc1-lymphocytes followed by CD4 Th1-lymphocytes producing mainly IFN-γ. The data obtained in the present study suggest that antigen-presenting cells play an important role in the in situ immune response through the production of proinflammatory cytokines, directing the cellular immune response preferentially to the Th1 and Tc1 types in NUCL caused by L. (L.) infantum chagasi.

Leishmania (L.) infantum chagasi isolated from skin lesions of patients affected by non-ulcerated cutaneous leishmaniasis lead to visceral lesion in hamsters.

In Central America, Leishmania (L.) infantum chagasi infection causes visceral leishmaniasis (VL) and non-ulcerated cutaneous leishmaniasis (NUCL). The aim of the present study was to evaluate the course of an experimental infection in hamsters caused by L. (L.) infantum chagasi isolated from patients affected by NUCL compared with a strain isolated from a patient with VL. Stationary phase parasites in culture were inoculated through subcutaneous and intraperitoneal routes in hamsters. Following the post-infection times, a histopathological study, parasite load and cytokine determination in skin from the cutaneous inoculation site and viscera were performed. Animals subcutaneously infected with the different strains did not develop macroscopic lesions at the inoculation site, and the histopathological changes in the dermis were very slight. Regarding the histopathological study of the viscera, we observed the portal mononuclear inflammatory infiltrate, the presence of nodules in the hepatic parenchyma and the proliferation of macrophages in the spleen, which increased over the infection course. Overall, the parasite load in the liver and spleen and in the total IgG titres in the sera of infected hamster showed an increase with the time of infection, regardless of the route of inoculation. Regarding cellular immunity, we did not observe an increase or decrease in pro- and anti-inflammatory cytokines compared to the healthy control, except for IL-10, which was evident in the infected animals. The data showed that strains isolated from NUCL cause visceral lesions in the hamsters regardless of the route of inoculation, and they were similar to parasites isolated from VL humans.

Can domestic dogs be considered a good reservoir of Leishmania (L.) infantum chagasi in an endemic area of nonulcerated cutaneous leishmaniasis in Southern Honduras?

ABSTRACT Dogs are considered to be the main domestic reservoir associated with the transmission of Leishmania (L.) infantum chagasi to humans in endemic areas of visceral leishmaniasis in America. However, little is known about the role of canines as a source of infection in endemic areas of nonulcerated cutaneous leishmaniasis (NUCL). Therefore, the objective of the present study was to investigate the role of dogs as a possible reservoir of the parasite in Southern Honduras. Dogs (n = 107) living with individuals affected by NUCL were clinically examined and biological material was collected for parasitological and immunological diagnosis. Most animals showed a healthy appearance and a few presented slight weight loss (64%), alopecia (7%), onychogryphosis (5%) and skin lesions (1%). The overall seroprevalence of Leishmania infection based on the DDP ® quick test and/or in-house ELISA serological test was 41%. The presence of the parasite's DNA was confirmed in 94% of the dogs; however, the average parasite load in the buffy coat was low at 6.09 parasites/µL, ranging between 0.221 and 50.2. The skin of seropositive dogs examined by histopathology using paraffin sections stained by hematoxylin and immunohistochemistry did not show cutaneous lesions or parasite amastigotes. Based on the absence of parasites in the skin and the low parasite load detected in the buffy coat, it seems that the dog does not represent a good source of infection for the vector in the endemic area of NUCL transmission in Southern Honduras. Other domestic and/or wild animals should be investigated.

A systematic review and meta-analysis of unmet needs for healthcare and long-term care among older people.

BACKGROUND: The absolute number of older individuals needing medical care and long-term care (LTC) is increasing globally due to the growing ageing population. However, it is uncertain who and what proportion of the population has access to care. Therefore, a systematic review and meta-analysis of the prevalence and reasons for unmet needs for healthcare and long-term care among older people, 65 years old and above, across countries was conducted. METHODS: An information specialist performed a comprehensive search of four major databases (PubMed, EMBASE, Web of Science, and CINAHL) from inception to June 2020 without restrictions on language and date. We did random-effects meta-analysis to obtain pooled prevalence. We stratified the meta-analysis by reasons for unmet need categorized by barrier dimension (availability, accessibility, affordability, and acceptability), survey year, geographic location, and socio-demographic characteristics of the older individual. RESULTS: After screening 3912 articles, we included 101 studies published between 1996 and 2020. Of the 101 studies, 87 studies reported unmet healthcare needs and 14 studies reported unmet LTC needs. Overall, 10.4% (95% CI, 7.3-13.9) of the older population had unmet needs for healthcare. The common reasons for unmet healthcare needs were cost of treatment, lack of health facilities, lack of/conflicting time, health problem not viewed as serious, and mistrust/fear of provider. A significant variation in pooled prevalence of unmet healthcare needs due to cost was found by gender (male [10.9, 95% CI, 8.9-13.1] vs female [14.4, 95% CI, 11.8-17.3]), educational level (primary or less [13.3, 95% CI, 9.6-17.6] vs higher [7.5, 95% CI, 5.9-9.3]), self-reported health (poor [23.2, 95% CI, 18.8-27.8] vs good [4.4, 95% CI, 3.4-5.5]), insurance status (insured [9.0, 95% CI, 7.5-10.6] vs uninsured [27.7, 95% CI, 24.0-31.5]), and economic status of population (poorest [28.2, 95% CI, 14.1-44.9] vs richest [7.1, 95% CI, 3.8-11.3]). One in four (25.1, 95% CI, 17.1-34.2) older people had unmet needs in LTC. Rural residents had a higher prevalence of unmet needs in LTC compared to their urban counterparts. CONCLUSION: With the population ageing globally, it is necessary to improve access to health care and LTC for older people. Ensuring affordability of health services, reducing geographical barriers, and improving acceptability, will be critical in reducing unmet need. Unmet needs for healthcare were concentrated in population with no education, poor economic group, outpatient health facility user, and uninsured group. With education and economic-based inequalities at the forefront, all countries should focus on improving access to health services by reducing the burden related to healthcare costs.

Unaffordability of COVID-19 tests: assessing age-related inequalities in 83 countries.

BACKGROUND: Diagnostic testing for SARS-CoV-2 is critical to manage the pandemic and its different waves. The requirement to pay out-of-pocket (OOP) for testing potentially represents both a financial barrier to access and, for those who manage to make the payment, a source of financial hardship, as they may be forced to reduce spending on other necessities. This study aims to assess age-related inequality in affordability of COVID-19 tests. METHODS: Daily data from the Global COVID-19 Trends and Impact Survey among adult respondents across 83 countries from July 2020 to April 2021 was used to monitor age-related inequalities across three indicators: the experiences of, first, reducing spending on necessities because of paying OOP for testing, second, facing financial barriers to get tested (from January to April 2021), and third, having anxiety related to household finance in the future. Logistic regressions were used to assess the association of age with each of these. RESULTS: Among the population ever tested, the adjusted odds of reducing spending on necessities due to the cost of the test decreased non-linearly with age from 2.3 [CI95%: 2.1-2.5] among ages 18-24 to 1.6 [CI95%: 1.5-1.8] among ages 45-54. Among the population never tested, odds of facing any type of barrier to testing were highest among the youngest age group 2.5 [CI95%:2.4-2.5] and decreased with age. Finally, among those reporting reducing spending on necessities, the odds of reporting anxiety about their future finances decreased non-linearly with age, with the two younger groups being 2.4-2.5 times more anxious than the oldest age group. Among those reporting financial barriers due to COVID-19 test cost, there was an inverse U-shape relationship. CONCLUSIONS: COVID-19 testing was associated with a reduction in spending on necessities at varying levels by age. Younger people were more likely to face financial barrier to get tested. Both negative outcomes generated anxiety across all age-groups but more frequently among the younger ones. To reduce age-related inequalities in the affordability of COVID-19 test, these findings support calls for exempting everyone from paying OOP for testing and, removing other type of barriers than financial ones.

Deconstructing Gender Differences in Experienced Well-Being Among Older Adults in the Developing World: The Roles of Time Use and Activity-Specific Affective Experiences.

Due to declining fertility rates and increasing longevity, the world is growing older. Improving the quality of life of older adults, and not merely preventing deaths, is thus becoming an important objective of public policies. It is, therefore, urgent to understand the key dimensions of older adults' subjective well-being as well as their main drivers. Women represent a large proportion of the older population, and existing evidence suggests that they may be particularly vulnerable, especially in the developing world. Analyzing potential gender differences in experienced well-being in older adults is hence crucial. We exploit information on time use and activity-specific emotional experiences from the abbreviated version of the day reconstruction method contained in the WHO Study on Global Ageing and Adult Health (SAGE), focusing on five developing countries. We first quantify gender differences in experienced well-being among older adults, which we then deconstruct into corresponding differences in time use and activity-specific net affects. Adjusting for age only, our results indicate a gender gap in experienced well-being in favor of men. Yet, adjusting for additional individual characteristics and life circumstances beyond age weakens this association. Illustrative counterfactual analyses further suggest that gender differences in activity-specific net affects appear more important than differences in time use for explaining the disadvantage of older women. Our results suggest that women's lower affect in most activities is linked to the conditions under which these activities are performed, and in particular to the higher level of disability of older women compared to men of the same age.

Scaling patterns of cerebellar petrosal lobules in Euarchontoglires: Impacts of ecology and phylogeny.

The petrosal lobules (in whole or part homologous with the paraflocculi) of the cerebellum regulate functions associated with vision including smooth pursuit and velocity control of eye movements, suggesting a possible relationship between the petrosal lobules and behavioral adaptation. Previous studies have produced diverging conclusions regarding the lobules' ecological signal. The current study examines lobule scaling within an ecologically diverse but phylogenetically constrained sample of extant mammals to determine whether ecology influences relative petrosal lobule size. Using the endocasts of 140 Euarchontoglires (Primates, Scandentia, Dermoptera, Lagomorpha, Rodentia), petrosal lobule size was evaluated relative to endocranium and body size, accounting for phylogenetic relationships and ecology (locomotor behavior, diet, activity pattern). Results show a strong positive relationship between lobule size and both endocranial volume and body mass. Phylogeny is a major factor in the scaling of the petrosal lobules, with significant differences in relative size identified between orders and suborders. Concerning ecology, fossorial taxa were found to have significantly smaller petrosal lobules relative to body mass compared to other locomotor groups across Euarchontoglires. The small lobules possessed by this group may reflect an adaptation related to reduced visual reliance. In contrast to previous research, no relationship was identified between relative lobule size and any other ecological variables. While variation in relative lobule size may be adaptively significant in some groups (i.e., fossorial species), it is critical to study the evolution of petrosal lobule size within a narrow phylogenetic scope, with inclusion of fossil material to inform our understanding of evolutionary trajectories.

Intervención educativa en pacientes con diabetes mellitus tipo II / Educational intervention in patients with type II diabetes mellitus

RESUMEN Introducción: El autocuidado constituye un elemento primordial en el control de la actividad clínica de los pacientes con diabetes mellitus tipo II. Objetivo: Realizar una intervención educativa para aumentar el nivel de conocimiento de los pacientes diabéticos sobre el autocuidado de la enfermedad. Métodos: Se realizó una investigación aplicada, cuasi experimental, donde el universo estuvo constituido por 115 pacientes con diagnóstico conformado de diabetes mellitus tipo II. La muestra quedó conformada por 90 pacientes; se determinaron el nivel de conocimiento y de control glucémico de los pacientes, sus características y las de la enfermedad. Se utilizó el cuestionario Diabetes Knowledge Questionnaire para determinar el nivel de conocimiento sobre autocuidado. Se aplicó la prueba no paramétrica de McNemar para identificar cambios provocados por la intervención educativa implementada. Resultados: Promedio de edad de 53,87 años con predominio de pacientes femeninas (73,33 %). Al inicio de la investigación predominaron pacientes con sobrepeso (53,33 %), complicaciones de la diabetes (68,89 %), nivel de conocimiento entre regular y malo (60,0 %) y control glucémico entre admisible e inadecuado (72,22 %). Después de implementado la intervención educativa el 81,33 % presentó nivel de conocimiento entre bueno y excelente y 56,56 % de los pacientes presentó un control glucémico entre adecuado y normal. Conclusiones: Se concluye que la intervención educativa implementada provocó cambios significativos en el aumento del nivel de conocimiento de los pacientes sobre autocuidado de la diabetes.

ABSTRACT Introduction: Self-care is an essential element in the control of clinical activity in patients with type II diabetes mellitus. Objective: To carry out an educational intervention to increase the level of knowledge of diabetic patients about self-care of the disease. Methods: An applied, quasi-experimental research was carried out; where the universe consisted of 115 patients with a confirmed diagnosis of type II diabetes mellitus. The sample was made up of 90 patients; the level of knowledge and glycemic control of the patients, their characteristics and those of the disease were determined. The Diabetes Knowledge Questionnaire was used to determine the level of knowledge about self-care. McNemar's non-parametric test was applied to identify changes caused by the educational intervention implemented. Results: Average age of 53.87 years with a predominance of females (73.33%). At the beginning of the investigation, overweight patients (53.33%), diabetes complications (68.89%), knowledge level between fair and bad (60.0%) and glycemic control between admissible and inadequate (72.22%) predominated. After implementing the educational intervention, 81.33% presented a level of knowledge between good and excellent and 56.56% of the patients presented a glycemic control between adequate and normal. Conclusions: It is concluded that the educational intervention implemented caused significant changes in the increase in the level of knowledge of patients about diabetes self-care.

Comparative Genomic Analyses of New and Old World Viscerotropic Leishmanine Parasites: Further Insights into the Origins of Visceral Leishmaniasis Agents.

Visceral leishmaniasis (VL), also known as kala-azar, is an anthropozoonotic disease affecting human populations on five continents. Aetiologic agents belong to the Leishmania (L.) donovani complex. Until the 1990s, three leishmanine parasites comprised this complex: L. (L.) donovani Laveran & Mesnil 1903, L. (L.) infantum Nicolle 1908, and L. (L.) chagasi Lainson & Shaw 1987 (=L. chagasi Cunha & Chagas 1937). The VL causal agent in the New World (NW) was previously identified as L. (L.) chagasi. After the development of molecular characterization, however, comparisons between L. (L.) chagasi and L. (L.) infantum showed high similarity, and L. (L.) chagasi was then regarded as synonymous with L. (L.) infantum. It was, therefore, suggested that L. (L.) chagasi was not native to the NW but had been introduced from the Old World by Iberian colonizers. However, in light of ecological evidence from the NW parasite's enzootic cycle involving a wild phlebotomine vector (Lutzomyia longipalpis) and a wild mammal reservoir (the fox, Cerdocyon thous), we have recently analyzed by molecular clock comparisons of the DNA polymerase alpha subunit gene the whole-genome sequence of L. (L.) infantum chagasi of the most prevalent clinical form, atypical dermal leishmaniasis (ADL), from Honduras (Central America) with that of the same parasite from Brazil (South America), as well as those of L. (L.) donovani (India) and L. (L.) infantum (Europe), which revealed that the Honduran parasite is older ancestry (382,800 ya) than the parasite from Brazil (143,300 ya), L. (L.) donovani (33,776 ya), or L. (L.) infantum (13,000 ya). In the present work, we have now amplified the genomic comparisons among these leishmanine parasites, exploring mainly the variations in the genome for each chromosome, and the number of genomic SNPs for each chromosome. Although the results of this new analysis have confirmed a high genomic similarity (~99%) among these parasites [except L. (L.) donovani], the Honduran parasite revealed a single structural variation on chromosome 17, and the highest frequency of genomic SNPs (more than twice the number seen in the Brazilian one), which together to its extraordinary ancestry (382,800 ya) represent strong evidence that L. (L.) chagasi/L. (L.) infantum chagasi is, in fact, native to the NW, and therefore with valid taxonomic status. Furthermore, the Honduran parasite, the most ancestral viscerotropic leishmanine parasite, showed genomic and clinical taxonomic characteristics compatible with a new Leishmania species causing ADL in Central America.

Evaluation of systemic immunity in atypical cutaneous leishmaniasis caused by Leishmania (L.) infantum chagasi.

In some central-American countries, Leishmania (L.) infantum chagasi infection can cause non-ulcerated or atypical cutaneous leishmaniasis (NUCL) in addition to the classic clinical form, visceral leishmaniasis (VL). Little is known about the host-parasite relationship that can contribute to the determination of one or another clinical form. The present study had the objective to evaluate the humoral and cellular immunity in the sera of individuals affected by NUCL to improve the comprehension of this atypical host-parasite interaction. Based on clinical and laboratory diagnosis, serum of 80 individuals was collected to evaluate the cytokines and immunoglobulins profile of NUCL (n = 47), VL patients (n = 5), and negative controls (n = 28). Cytokines were detected using Cytokine Bead Array (CBA) Human Th1/Th2/Th17 kit according to the manufacturer's instructions; class (IgG and IgM), and subclass of (IgG1 and IgG2) immunoglobulins was evaluated by ELISA using specific antigens. The concentration of TNF-α, IFN-γ, IL-2 and IL-4 cytokines in NUCL, VL and control was present below the detection threshold of CBA kit. IL-6, IL-10 and IL-17A cytokines was lower in NUCL compared to LV patients. Regarding to immunoglobulins, NUCL patients produced 4.0 times more IgG than the control, while VL patients produced 6.6 times more; and IgM level was 1.6 times higher in NUCL and 2.6 times in VL patients compared to the control. Concerning the immunoglobulins subclass, only VL patients showed positive reaction for IgG1, and IgG2 did not show positive reaction among the groups. The results showed a weak cellular and humoral systemic immune response in NUCL patients.

Whole-Genome Sequencing of Leishmania infantum chagasi Isolates from Honduras and Brazil.

This work reports on the whole-genome sequencing of Leishmania infantum chagasi from Honduras (Central America) and Brazil (South America).

[Sustainable Development Goals: impact of lack of financial protection in health in Latin American and Caribbean countriesObjetivos de Desenvolvimento Sustentável: impacto da falta de proteção financeira em saúde nos países da América Latina e do Caribe]. / Objetivos de Desarrollo Sostenible: impacto de la falta de protección financiera en salud en países de América Latina y el Caribe.

OBJECTIVE: To determine the impact of household out-of-pocket health spending, financial protection levels, and their inequality according to relevant variables in the countries of the Region; to investigate their evolution and relationship with health system services utilization. METHODS: Comparative descriptive information was compiled on out-of-pocket spending, its incidence in the population, and its weight and contribution to household consumption. Financial protection indicators for the national level and their distribution by quintiles of total household consumption and by gender are presented, and compared to an indicator of service coverage. RESULTS: Out-of-pocket spending and financial protection indicators are deficient but demonstrate differences among countries. The composition of health expenditure is identified for a subset of countries and significant gradients are seen when inequalities are studied. Changes over time and possible associations with service coverage levels are shown for several cases. DISCUSSION: Lack of financial protection affects a large part of the population. Additionally, there are groups of countries with greater difficulties than others, where a preponderance of spending is on medicines, and there is greater exposure of groups in conditions of vulnerability, such as the poorest and women, indicating great inequity. Policies of some countries that can be associated with improvement in financial protection are identified. To replace out-of-pocket spending, which is a barrier to access, countries need to increase public spending by financing health systems undergoing transformation toward universal health.

OBJETIVO: Determinar o impacto dos gastos diretos em saúde nas famílias, os níveis de proteção financeira e sua desigualdade, de acordo com variáveis relevantes nos países da Região; investigar sua evolução e sua relação com a utilização dos serviços do sistema de saúde. MÉTODOS: Foram coletadas informações descritivas comparativas sobre gastos diretos em saúde, sua incidência na população e seu peso e composição no consumo familiar. Os indicadores de proteção financeira são apresentados em nível nacional, estratificados por quintis de consumo total das famílias e por gênero, e são comparados com um indicador de cobertura de serviço. RESULTADOS: Os indicadores de gastos diretos e proteção financeira são limitados, mas demonstram diferenças entre os países. Foi possível identificar a composição dos gastos com saúde para um subgrupo deles, observando-se gradientes significativos quando as desigualdades são estudadas. Em alguns casos, observam-se também mudanças ao longo do tempo e possíveis associações com os níveis de cobertura de serviço. DISCUSSÃO: A falta de proteção financeira atinge grande parte da população. Foram observados grupos de países com mais dificuldades do que outros, com preponderância de gastos com medicamentos e maior exposição de grupos em situação de vulnerabilidade, como os mais pobres e as mulheres, o que denota alto nível de iniquidade. Em alguns países foram identificadas políticas que podem estar associadas à evolução da proteção financeira. Para substituir os gastos diretos, que constituem uma barreira ao acesso, os países precisam aumentar o gasto público, financiando os sistemas de saúde numa transformação rumo à saúde universal.

Challenges and experiences in linking community level reported out-of-pocket health expenditures to health provider recorded health expenditures: Experience from the iHOPE project in Northern Ghana.

Out of pocket health payment (OOPs) has been identified by the System of Health Accounts (SHA) as the largest source of health care financing in most low and middle-income countries. This means that most low and middle-income countries will rely on user fees and co-payments to generate revenue, rationalize the use of services, contain health systems costs or improve health system efficiency and service quality. However, the accurate measurement of OOPs has been challenged by several limitations which are attributed to both sampling and non-sampling errors when OOPs are estimated from household surveys, the primary source of information in LICs and LMICs. The incorrect measurement of OOP health payments can undermine the credibility of current health spending estimates, an otherwise important indicator for tracking UHC, hence there is the need to address these limitations and improve the measurement of OOPs. In an attempt to improve the measurement of OOPs in surveys, the INDEPTH-Network Household out-of-pocket expenditure project (iHOPE) developed new modules on household health utilization and expenditure by repurposing the existing Ghana Living Standards Survey instrument and validating these new tools with a 'gold standard' (provider data) with the aim of proposing alternative approaches capable of producing reliable data for estimating OOPs in the context of National Health Accounts and for the purpose of monitoring financial protection in health. This paper reports on the challenges and opportunities in using and linking household reported out-of-pocket health expenditures to their corresponding provider records for the purpose of validating household reported out-of-pocket health expenditure in the iHOPE project.

Objetivos de Desarrollo Sostenible: impacto de la falta de protección financiera en salud en países de América Latina y el Caribe

[RESUMEN]. Objetivo. Determinar el impacto del gasto de bolsillo en salud en los hogares, los niveles de protección financiera y su desigualdad según variables relevantes en países de la Región. Se indaga su evolución y relación con el uso de servicios del sistema de salud. Métodos. Se recopila información descriptiva comparada acerca del gasto de bolsillo, su incidencia en la población, y su peso y composición en el consumo de los hogares. Se presentan indicadores de protección financiera en el nivel nacional y su distribución por quintiles de consumo total de hogares y por género. Se contrastan con un indicador de cobertura de servicios. Resultados. Los indicadores de gasto de bolsillo y protección financiera son deficientes pero diferenciados entre los países. Se identifica la composición del gasto en salud para un subgrupo de ellos y existen gradientes significativos cuando se estudian las desigualdades. Para algunos casos, se muestran cambios en el tiempo y posibles asociaciones con los niveles de cobertura de servicios. Discusión. La desprotección financiera afecta a una gran parte de la población, se configuran grupos de países con dificultades mayores que otros, con preponderancia de gasto en medicamentos y exposición mayor de grupos en situaciones de vulnerabilidad, como los más pobres y las mujeres, lo que denota una gran inequidad. Se identifican políticas de algunos países que pueden asociarse con la evolución de la protección financiera. Para reemplazar el gasto de bolsillo, barrera para el acceso, los países necesitan aumentar el gasto público mediante el financiamiento de los sistemas de salud en transformación hacia la salud universal.

[ABSTRACT]. Objective. To determine the impact of household out-of-pocket health spending, financial protection levels, and their inequality according to relevant variables in the countries of the Region; to investigate their evolution and relationship with health system services utilization. Methods. Comparative descriptive information was compiled on out-of-pocket spending, its incidence in the population, and its weight and contribution to household consumption. Financial protection indicators for the national level and their distribution by quintiles of total household consumption and by gender are presented, and compared to an indicator of service coverage. Results. Out-of-pocket spending and financial protection indicators are deficient but demonstrate differences among countries. The composition of health expenditure is identified for a subset of countries and significant gradients are seen when inequalities are studied. Changes over time and possible associations with service coverage levels are shown for several cases. Discussion. Lack of financial protection affects a large part of the population. Additionally, there are groups of countries with greater difficulties than others, where a preponderance of spending is on medicines, and there is greater exposure of groups in conditions of vulnerability, such as the poorest and women, indicating great inequity. Policies of some countries that can be associated with improvement in financial protection are identified. To replace out-of-pocket spending, which is a barrier to access, countries need to increase public spending by financing health systems undergoing transformation toward universal health.

[RESUMO]. Objetivo. Determinar o impacto dos gastos diretos em saúde nas famílias, os níveis de proteção financeira e sua desigualdade, de acordo com variáveis relevantes nos países da Região; investigar sua evolução e sua relação com a utilização dos serviços do sistema de saúde. Métodos. Foram coletadas informações descritivas comparativas sobre gastos diretos em saúde, sua incidência na população e seu peso e composição no consumo familiar. Os indicadores de proteção financeira são apresentados em nível nacional, estratificados por quintis de consumo total das famílias e por gênero, e são comparados com um indicador de cobertura de serviço. Resultados. Os indicadores de gastos diretos e proteção financeira são limitados, mas demonstram diferenças entre os países. Foi possível identificar a composição dos gastos com saúde para um subgrupo deles, observando-se gradientes significativos quando as desigualdades são estudadas. Em alguns casos, observam-se também mudanças ao longo do tempo e possíveis associações com os níveis de cobertura de serviço. Discussão. A falta de proteção financeira atinge grande parte da população. Foram observados grupos de países com mais dificuldades do que outros, com preponderância de gastos com medicamentos e maior exposição de grupos em situação de vulnerabilidade, como os mais pobres e as mulheres, o que denota alto nível de iniquidade. Em alguns países foram identificadas políticas que podem estar associadas à evolução da proteção financeira. Para substituir os gastos diretos, que constituem uma barreira ao acesso, os países precisam aumentar o gasto público, financiando os sistemas de saúde numa transformação rumo à saúde universal.

Macrophage Polarization in the Skin Lesion Caused by Neotropical Species of Leishmania sp.

Macrophages play important roles in the innate and acquired immune responses against Leishmania parasites. Depending on the subset and activation status, macrophages may eliminate intracellular parasites; however, these host cells also can offer a safe environment for Leishmania replication. In this sense, the fate of the parasite may be influenced by the phenotype of the infected macrophage, linked to the subtype of classically activated (M1) or alternatively activated (M2) macrophages. In the present study, M1 and M2 macrophage subsets were analyzed by double-staining immunohistochemistry in skin biopsies from patients with American cutaneous leishmaniasis (ACL) caused by L. (L.) amazonensis, L. (V.) braziliensis, L. (V.) panamensis ,and L. (L.) infantum chagasi. High number of M1 macrophages was detected in nonulcerated cutaneous leishmaniasis (NUCL) caused by L. (L.) infantum chagasi (M1 = 112 ± 12, M2 = 43 ± 12 cells/mm2). On the other side, high density of M2 macrophages was observed in the skin lesions of patients with anergic diffuse cutaneous leishmaniasis (ADCL) (M1 = 195 ± 25, M2 = 616 ± 114), followed by cases of localized cutaneous leishmaniasis (LCL) caused by L. (L.) amazonensis (M1 = 97 ± 24, M2 = 219 ± 29), L. (V.) panamensis (M1 = 71 ± 14, M2 = 164 ± 14), and L. (V.) braziliensis (M1 = 50 ± 13, M2 = 53 ± 10); however, low density of M2 macrophages was observed in NUCL. The data presented herein show the polarization of macrophages in skin lesions caused by different Leishmania species that may be related with the outcome of the disease.